Evaluation Of Female Reproductive Toxicity Of Hydroalcoholic Extract Of Cassia Occidentalis (CO-A002) In Rodents

Abstract

Objectives: The aim of present study is to evaluate the toxicity of the hydroalcohlic extract of leaves of Cassia occidentals, (Fabaceae), on reproductive parameters (on theestrous cycle, reproductive performance, post-natal growth and offspring survival of rats). Here four groups of not-pregnant Wistar rats received distilled water and the doses 250, 500 and 1000 mg/kg of plant extract for thirty days, at the end of which they were examined as to the frequency of their phases in toxicity on the estrous cycle.
Methodology: Thirty-two (32) female wistar rats with consecutive 4 to 6 days estrous cycle were given distilled water (as control) and CO-A002 at 250, 500 and 1000 mg/kg dose daily by gavaging ten days prior to mating, mating (a maximum period of ten days), gestation and lactation periods of seven days. Dams and fetuses were sacrificed on day seven postnatal. Four groups of not-pregnant Wistar rats received distilled water and the doses 250, 500 and 1000mg/kg of plant extract in toxicity on the estrous cycle at the end of which they were examined as to the frequency of their phases in toxicity on the estrouscycle.
Results:CO-A002extracts did not bring change in the estrous cycle and general health of all wistar rats. All the animals
proceed towards successful mating and pregnancies. There was no significant alteration in the duration of pregnancy. Moreover, pregnant rats delivered pups normally. Statistically no test agent-related changes in the body weight of female wistar rats, number of implantations, litter size and pup body weights were observed. Other parameters measured include pup male: female ratio, live birth index, pup viability index and percentage of implantation death which also showed no significant change.
Conclusion: The present findings reveal that hydroalcohlic extract of Cassia Occidentalis didn’t exhibit any remarkable
reproductive toxicity.