Genotypic Characterization Of Virulence Factor Afa, Papc, Sfa And Molecular Detection Of Bla TEM, Bla SHV, Bla CTX-M Genes According To Phytogenetic Group In Extended Spectrum Β- Lactamase (ESBL) Escherichia Coli Strains Isolated From Urinary Tract Infect

Abstract

Aim: Drug resistance among gram positive Escherichia coli bacteria is a worldwide challenge. Due to the importance of antibiotic resistant E.coli strain in hospital acquired Urinary Tract Infection. The present study was determine the genotypic characterisation detection of virulence factor, molecular phylogenetic group and ESBL genes producing isolated obtained from hospitalized patients in Kerala

Material and method: In total (123/ 65) Escherichia coli isolated from years of April 2022 to May 2023 were collected from various infections genotypic identification of bacterial isolated was performed using standard method. Genotypic screening of virulence factor afa, papC, sfa and molecular detection ESBL genes bla_TEM, bla_SHV, bla_CTX-M genes was also performed by the PCR & phylogenetic group method.

Results: The number of virulence factor genes afa, papC, sfa, eight isolated were multiplex PCR using amplification specific genes, five different UTIs genotypes patterns were observed and bla_TEM, bla_SHV, bla_CTX-M was the most prevalent ESBL genotypes TEM (-890.46), SHV (1162.03), CTX-M (-2778.42) and nucleotides 41, 23, 50. This may be because of the reduction in initial mass taken for RNA upon antibiotic treatment. Expression of CTX-M and TEM was comparatively higher than SHV gene.

Conclusion: The emergence of ESBL – producing E. coli isolates with high antibiotic – resistant rates to commonly used antibiotics and increased predominance of major gene type’s bla_CTX-M is a serious concern to the clinicians and microbiologists. Periodic monitoring of antibiotic susceptibility and associated genes would help guide.