SDS-PAGE Analysis of Protein Extracts From Hybanthus enneaspermus (L.) F. Muell: Regional Variations In Molecular Profiles

Abstract

Belonging to the Violaceae family, Hybanthus enneaspermus (L.) F. Muell is a petite perennial herbaceous plant found in tropical and subtropical regions worldwide, renowned for its medicinal applications. In this current investigation, the focus was on isolating proteins from this plant in four distinct regions (Marthandam, Petchiparai, Nagercoil, and Chothavilai) using SDS-PAGE. Proteins, being a class of biological macromolecules, present well-characterized physico-chemical properties compared to nucleic acids, simplifying their extraction and characterization. The research highlighted a higher protein concentration in the Marthandam region (2.63 mg/ml of the sample). SDS-PAGE analysis revealed diverse protein bands with varying molecular weights across the four regions, shedding light on the regional variations in the protein profile of Hybanthus enneaspermus (L.) F. Muell.