SDS-PAGE Analysis of Protein Extracts From Hybanthus enneaspermus (L.) F. Muell: Regional Variations In Molecular Profiles

T. C. Beaula Stary; S. Uma Devi; Beena Lawrence; G. Johnsi Christobel

doi:10.53555/sfs.v10i1.2245

Authors

T. C. Beaula Stary
S. Uma Devi
Beena Lawrence
G. Johnsi Christobel

DOI:

https://doi.org/10.53555/sfs.v10i1.2245

Keywords:

Hybanthus enneaspermus (L.) F. Muell, SDS Page, Proteins, Gel electrophoresis

Abstract

Belonging to the Violaceae family, Hybanthus enneaspermus (L.) F. Muell is a petite perennial herbaceous plant found in tropical and subtropical regions worldwide, renowned for its medicinal applications. In this current investigation, the focus was on isolating proteins from this plant in four distinct regions (Marthandam, Petchiparai, Nagercoil, and Chothavilai) using SDS-PAGE. Proteins, being a class of biological macromolecules, present well-characterized physico-chemical properties compared to nucleic acids, simplifying their extraction and characterization. The research highlighted a higher protein concentration in the Marthandam region (2.63 mg/ml of the sample). SDS-PAGE analysis revealed diverse protein bands with varying molecular weights across the four regions, shedding light on the regional variations in the protein profile of Hybanthus enneaspermus (L.) F. Muell.

Author Biographies

T. C. Beaula Stary

Reg. No: 19133112262007, Research Scholar, Department of Botany, Nesamony Memorial Christian College, Marthandam
S. Uma Devi

Assistant Professor, Department of Botany, Sree Ayyappa College for Women, Chunkankadai
Beena Lawrence

Associate Professor, Department of Botany, Women’s Christian College, Nagercoil.
G. Johnsi Christobel

Associate Professor and Head, Department of Botany, Nesamony Memorial Christian College, Marthandam.

Affiliated to Manonmaniam Sundaranar University, Abhisekapatti, Tirunelveli, Tamil Nadu, India