Exploring The Therapeutic Potential: In-Vitro Assessment of Antioxidant and Anti-Inflammatory Activity Of methanolic extract from Tabernaemontana divaricata Leaves

Abstract

Introduction: Medicinal plants have various applications in the field of medical sciences. Plants are wide source of numerous phyto-constituents which have been proved to have multiple health benefits. Literature says that leaves of Tabernaemontana divaricata are loaded with such phyto-chemicals which can be a target in case of treatment of various infectious diseases. In Ayurvedic Medicine, the juice form of flower buds is mixed with oil and applied to the skin to treat inflammation. is also used in dental care, for scabies, as cough medicine and for eye ailments

Aim: To evaluate in-vitro antioxidant and anti-inflammatory activity of the methanolic extract of the leaves of Tabernaemontana divaricate.

Method: Methanolic extraction of the leaves of Tabernaemontana divaricata was made by using maceration method for 5 days and solvent was evaporated using rotary evaporator. To evaluate the anti-oxidant property, total phenolic content and total flavonoid content was measured against gallic acid and quercetin as reference. The in-vitro anti-inflammatory study was performed using techniques like heat induced hemolysis, hypotonicity induced hemolysis, protein denaturation study and protease inhibitory activity using goat blood cell.

Result: The total flavonoids content and total phenolic content were 38.57μg/ml and 45.48μg/ml respectively. The IC₅₀ (Inhibitory concentration 50%) value was measured for each anti-inflammatory study. The IC₅₀ value were 416 μg/ml, 166.66 μg/ml, 188 μg/ml and 280 μg/ml for the methods heat induced hemolysis, hypotonicity induced hemolysis, protein denaturation study and protease inhibitory activity respectively

Conclusion: In conclusion, the methanolic extract of Tabernaemontana divaricata leaves exhibited significant antioxidant and anti-inflammatory properties. The high content of flavonoids and phenolic compounds underscores its potential therapeutic value. The extract demonstrated promising inhibitory effects against various inflammation-inducing mechanisms, as evidenced by the low IC50 values in the in-vitro anti-inflammatory assays. These findings support the traditional uses of Tabernaemontana divaricata in treating inflammation-related conditions. Further research exploring its mechanisms of action and potential clinical applications is warranted