Antibacterial Effect Of Punica Granatum Acetone Leaf Extract Against Aeromonas Hydrophila Affecting Gold Fish Carassius Auratus

Abstract

In the present study, Aeromonas hydrophila, a bacteria isolated from infected goldfish (Carassius auratus), was tested for its antibacterial efficacy against Punica granatum leaf - acetone extract. Diffusion disc plates on agar were used to measure the antibacterial activity. Different concentrations (25 %, 50 %, 75 % and 100 %) of P. granatum leaf - acetone extract was tested against A. hydrophila. The P. granatum leaf – acetone extract was exhibited a significant level of inhibition against A. hydrophila. Maximum zone of clearance was measured after 24 hours of incubation at 37°C. The result of the present study revealed that the fish pathogen A. hydrophila was exhibited maximum zone of clearance (18 mm) at 100 % concentration of P.granatum leaf - acetone extract, where as the minimum zone of clearance      8 mm was recorded at 25 % of concentration. Fourier Transform Infra-Red Spectroscopy (FTIR) of     P. granatum leaf –acetone extract revealed the presence aliphatic, alkenes, nitrogen, carbonyl and hydroxyl groups. The phytochemical analysis of the P. granatum leaf – acetone extract showed the presence of tannins, carbohydrates, terpenoids, phenols, proteins and steroids. As per the result of the present study, it can be concluded that phytochemicals were identified in P. granatum leaf – acetone extract was effective against A. hydrophila, a microbial pathogen that has infected goldfish (C.auratus).