Protein Profiles Of Pacific White Shrimp (Litopenaeus Vannamei) And Hermit Crab, (Clibanarius Longitarsus) Before And After WSSV Infection

Abstract

The prevalence of White Spot Syndrome Virus (WSSV) infection in the cultured shrimps is a havoc and leads to devastation of the aquaculture industry. In this backdrop, the present study is designed to investigate the changes in protein profile before and after infection for the candidate species Litopenaeus vannamei. The hermit crab Clibanarius longitarsus was also chosen for this investigation as it is known for sturdy against viral infection. Hence, it is determined to undergo protein profile study in order to light up the path of drug design in the near future. Both the candidate species were confirmed for non-infectious using DNA based molecular diagnostic (PCR) method and subsequently the experiment was initiated in the laboratory by making infection with WSSV through feeding with WSSV-positive tissues. The infection was confirmed in the course of challenge test by collecting the hemolymph, isolating the DNA and amplifying using PCR, and running on gel electrophoresis. For further confirmation, SDS PAGE method has been performed for the healthy and WSSV infected Litopenaeus vannamei and Clibanarius longitarsus. The gel electrophoresis of SDS PAGE reveals the expression of Heat Shock Protein (HSP 60) which is a signal cascade that is expressed as an immune response. This
investigation on the proteomics showed promising results between the species of highly vulnerable Litopenaeus vannamei and asymptomatic Clibanarius longitarsus.