Characteristics of Meristics, Morphometrics, and Analysis of Cytochrome Oxidase Gen Fragmen Subunit 1 (Co1) Hampala Barb (Hampala Sp) from Jatigede Reservoir, Sumedang, West Java, Indonesia

Abstract

Hampala barb in Jatigede Reservoir was one of the native fish of the Cimanuk River that needed to be conserved. This fish was included in the IUCN Red List with the category of Least Concern. The purpose of this study was to collect information related to biometric (morphometric and meristic) and molecular characteristics of the platinum fish in the Jatigede Reservoir. Morphometric and meristic analysis results showed that Hampala barb originating from Jatigede Reservoir, both those with spot (HS) and those without (HNS), were both identified as Hammpala macrolepidota Kuhl and Van Hasselt, 1823. Analysis of nucleotides and amino acids from gene fragments Cytochrome Oxidase Subunit 1 (CO1) showed differences in composition between fish samples that had spots (HS) and those that did not have spots (HNS). In the HS sample, the number of amino acids Leucine (Leu) was found the most, while Alanin (Ala) was present predominantly in the HNS sample. Meanwhile, the results of the BLAST analysis showed that the HS sample had a closeness to Hampala dispar (Acc No. MK448077.1) originating from Thailand, with a similarity percentage of 91.07%. Meanwhile, the HNS sample fish found no similarities to the CO1 database in Genbank, however, phylogenic analysis showed a close relationship between HS and HNS sample fish, and both were separate from other Hampala clade species in Genbank.