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In this study, a total of 82 (49♀♀, 33♂♂) Cyprinion kais samples from the same location in the river of Tigris were collected and ages of scales taken from front and upper section of lin lateral of dorsal fins of fishes were determined and photographed by an Olympus digital camera with Canon SX7 model binocular under the same conditions and then six landmarks were taken by using tpsDig ver. 2.32 software. Afterwards, procrustes analysis was conducted. After separating shapes and sizes, ANOVA, PCA, CVA/MANOVA and DFA analyses were performed. According to the results of the analyses, there was a significant difference between the samples in terms of gender and size (Size ANOVA: F= 50.6, p<.0001); at the same time the difference between samples in terms of shape was at the level of (Shape ANOVA: F= 3.92, p =0.0002); there was a significant difference between samples at different ages in terms of size (Size ANOVA: F= 44.08, p<.0001), however, no difference was found in terms of the shape of the samples; there was a significant difference between size (Size ANOVA: F= 17.87, p<.0001) of the samples but no difference was found between shape of them depending on the season. For gender, age and seasons, the first two components explain 61.4%, 63.6% and 63.4% of total variance, respectively. As a result, there was a difference between sizes of the C. kais species depending on gender, age of the fishes and the season; however, although there was a difference between shapes of the samples depending on gender variable, no difference was found depending on age of the fish and the season.