Impacts of Atrazine on some blood and biochemical indices in farmed Acipenser nudiventris

Abstract

In this study, the effects of the Atrazine on some blood biochemistry indices in
Acipenser nudiventris during a 96 hours period was studied. These examinations were
made by 4 treatments and each treatment repeated these exams three times with a blank,
and these were done on 135 Acipenser nudiventris with the average weight of 50 ±10 g.
The temperature, pH and hardness of the water and the dissolved oxygen were 24±1
oC,
8, 400 and 7 mg per liter, respectively. The amount of LC50
-96h of Atrazine was
estimated 32.01 mg per liter. The minimum and maximum level of albumin in
treatment 50 mg per liter was observed in the 24th and 48th hour and there was a
significant difference (p<0.05). The
cholesterol level in all concentrations showed
significant decrease in comparison with the blank in time. The
triglyceride had its
minimum amount in the concentration of 37 mg per liter, which had a significant
difference with respect to the blank and some other treatments (p<0.05). The
glucose
level in all the treatments in 24 hours showed no significant difference with the blank
treatment (p<0.05). As time went, the
hematocrit increased meaningfully with the
increase of the atrazine concentration, and the red blood cells (RBC) decreased
meaningfully in different treatments. The amount of the white blood cells (WBC) had
decreased meaningfully in the 24th and 48th hour in comparison to the blank. The
amount of hemoglobin showed meaningful tolerance in different times and
concentrations. Changes in the average cellular volume had a meaningful difference
with the increase of the atrazine dose and in different treatments and times. The average
changes in the hemoglobin corpuscular showed a meaningful difference in different
treatments and different times. The average of corpuscular hemoglobin in 24 hours had
a meaningful increase in the most of the treatment in comparison with the blank. In
some of the treatments the neutrophils have increased meaningfully with the increase of
concentration and with time passing by. Its minimum amount has been observed in 96
hours in the concentration of 50 mg per liter. In some of the treatments, with time going
by, the amount of the lymphocytes with the increase of the atrazine concentration has
increased meaningfully in comparison to the blank. The amount of neutrophils in the
24th and 48th hour showed meaningful increase with the increase of the atrazine
concentration and in time, in some of the treatments in comparison to the
blank. The
monocytes have increased meaningfully in time and with the increase of the atrazine
concentration. According to the table of the toxicity levels, atrazine is considered as
‘toxic’ for Acipenser nudiventris and we can use the changes in the desired indices as
tools in analyzing the situation in the pathobiology of fishes