Pharmacognostical Standardization, Formulation And Evaluation Of Carica Papaya L. Sparkling Water For Dengue Fever And Its Management

Abstract

The incidence of Dengue fever, a mosquito-borne viral illness, has escalated in recent years across various countries including Bangladesh, Philippines, Thailand, Brazil, and India, leading to substantial morbidity and mortality. Traditional remedies utilizing Carica papaya leaves in fresh form have shown promise in alleviating Dengue fever symptoms, particularly thrombocytopenia, a common complication. To address issues of stability and palatability of fresh Carica papaya leaves a novel dosage form utilizing carbonated fresh juice of Carica papaya leaves is proposed. Carbonation not only preserves the bioavailability of the active constituents but also enhances patient acceptability. This innovative approach offers a convenient and effective means of delivering Carica papaya leaf extract for Dengue fever management, potentially improving patient outcomes worldwide. The described DNA isolation and subsequent sequencing and BLAST analysis conclusively identified the sample as Carica papaya, affirming the reliability and efficacy of the employed methodology for genetic analysis and species identification. Different compositions of the mobile phase for HPTLC analysis were tested in order to obtain high resolution and reproducible peaks. Authentic markers of flavonol (quercetin) obtained commercially was cochromatographed. Blue brown colour zone was detected in UV derivatisation in the chromatogram which confirms the presence of flavonoid quercetin in the formulation. This novel formulation was standardized using modern sophisticated instuments like TLC, HPTLC, DNA barcoding and UV analysis. This formulation would be definitely helpful for the future mankind for better dengue management.