In Vitro Axillary Bud Multiplication Of An Important Medicinal Plant-Rubia Cordifolia L.

Abstract

An efficient protocol was established for micropropagation Rubia cordifolia by multiplying axillary buds from nodal explants. The excised nodal segments were inoculated onto MS medium supplemented with various concentrations and combinations of plant growth regulators. The best combination for the shoot and root proliferation of R. cordifolia was Murashige and Skoog’s medium fortified with 0.5 mg/L Kinetin. Each explant produced an average of 8 shoots within 80 days of incubation. The in vitro-developed plantlets were hardened and successfully established in pots, achieving a 95% survival rate. The organogenesis protocol developed in this study may enhance the clonal propagation and conservation of R. cordifolia, an important industrial and medicinal plant.