omparison of the effects of propolis and pollen extracts in the same concentrations on some biochemical and hematological parameters in rainbow trout (Oncorhynchus mykiss)

Abstract

Three different concentrations (10, 20 and 30 ppm) of the dry propolis and pollen extracts were applied in double distilled water. Biochemical and hematological parameters in blood of rainbow trout (Oncorhynchus mykiss) treated to various propolis and pollen concentrations (10, 20 and 30 ppm) for 96 h were determined.  Fish treated to 20 and 30 ppm of propolis extract presented significant increases (p<0.05) in glucose, triglycerides, total cholesterol levels and LDH activities as compared with the 10 ppm.  But, there were significant decreases in AST, ALP, erytrocyte and hemoglobin as compared with the 10 ppm. In groups administrated pollen extracts; there were significant decreases in the levels of urea and glucose in 10 and 20 ppm concentrations compared with 30 ppm (p<0.05). Significant changes in the erytrocyte counts and hematocrit values in blood of fish treated to 20 and 30 ppm pollen were observed compared to 10 ppm pollen group (p<0.05). The data showed that propolis and pollen have important positive effects on biochemical and hematological parameters at 10 ppm levels, whereas other concentrations appear to be unfavorable for blood of rainbow trouts. Due to antioxidant and preservative properties of propolis and pollen, they may not only prolong the physiological functions of some aquatic living organisms, but also contribute to the health benefit of consumers who consume aquatic animals.