Hptlc Fingerprint Profile of Glycosides from Hydroalcohol Extract Of Leaves of Holoptelea Integrifolia (Roxb.)

Abstract

India by the tribal people for it’s medicinal properties. Plants and plant-based products are the basis of many of the modern pharmaceuticals we use today for various ailments. High performance thin layer chromatography (HPTLC) is a valuable tool for the investigation of herbal products with respect to different aspects of their quality.

High Performance Thin Layer Chromatography (HPTLC) technique was utilized to find Glycosides from hydroalcohol exrtact of therapeutically and commercially beneficial leaves of Holoptelea integrifolia. Phytochemical screening was performed as per standard procedure followed by HPTLC analysis. HPTLC instrumentation of CAMAG was having Linomat V applicator (Switzerland). CAMAG Thin Layer Chromatography scanner IV with reflectance absorbance mode at 208 nm , Win CATS software (1.4.6 Camag) and tungstant lamp was utilized to perform densitometric scanning.Hydroalcohol extract of Holoptelea integrifolia appeared to give the presence of Glycosides, tannins, alkaloids, glycosides, flavonoids, phytosterols from preliminary phytochemical analysis. Our aim is to do HPTLC studies from hydroalcohol extract of leaves of Holoptelea integrifolia. Objective that were assessed are hydroalcohol extract of leaves of Holoptelea integrifolia. HPTLC studies revealed eleven multivalent phytochemicals (11 peaks) having Rf values in the increasing range of 0.13 to 0.90 Rf 0.90 has 35.67% concentration. Preliminary phytochemical analysis and Rf Values authenticated existence of Glycosides in hydroalcohol extract