Computational Analysis of Important Mirna and Gene Network Analysis of Braf Gene, A Key Regulator of Head and Neck Cancer

Abstract

BACKGROUND: Head and neck cancer refers to cancer of the mouth, throat, nose, salivary glands, oral malignancies, and other regions of the head and neck. BRAF is a human gene that codes for the B-Raf protein. The protein is known as serine/threonine-protein kinase B-Raf, and the gene is known as proto-oncogene B-Raf and v-Raf murine sarcoma viral oncogene homolog B.

AIM: To find key miRNAs and other genes that are strongly linked to the BRAF gene.

METHODS:  Target scan prediction of microRNA targets for BRAF gene in humans was

Carried out using portal.gdc.cancer.gov. Targets of the BRAF gene were identified using the program with default settings. The targets of the conserved miRNA. hsa-miR-6507-5p miRNA with a miR score of ≥0.99 were identified using the mirdb.org software program. Gene network analysis of BRAF was carried out by STRING database online server program. Target mRNAs were predicted using miRDB online server program. miRNA targets with a  target score of more than >90 were  considered for further analysis.

RESULTS: In this study it was observed that 49 miRNAs were targeted by the BRAF gene through miRDB analysis. Of these, two miRNAs (hsa-miR-6507 and hsa-miR-12136) were selected that had a target score of >99. NEK7, PLS3, KIAA0408, PTAR1 and NR1D2 were some of the genes found to have interactions with the BRAF gene.

CONCLUSION: Our research discovered key miRNAs that may have a role in head and neck cancer. Other interacting genes might help researchers better understand the carcinogenesis process in head and neck cancer.