Anti-proliferative and antimigratory potential of cinnamomum cassia bark extract on breast cancer cells

Abstract

Background: Breast cancer is the most prevalent cancer in women in the world, accounting for approximately 25% of all female malignancies, with a higher frequency in developed countries. Breast cancer develops when abnormal cells in the breast divide and multiply, turning it into a malignant tumor. Cinnamic acid derivatives have been shown to suppress breast cancer cells and also suppress proliferation and tumor progression.

Aim: The aim of the study is to assess the Anti- proliferative and antimigratory potential of cinnamomum cassia bark extract on breast cancer

cells.

Materials and methods: Estrogen-dependent (MCF-7) breast cancer cell lines were received from the NCCS, Pune, for cell line maintenance. Then, the Herbal Extract Preparation and Cell Viability (MTT) Assay were evaluated. Mcf7 cells (2×105 cells/well) were seeded into six-well culture plates for a morphology study , and cell migration examined using a scratch wound healing assay. A 200-l tip was used to scrape the cell monolayer in order to make a wound, and pictures of the results were taken. Then, Statistical analysis- All data obtained were analyzed by One Way ANOVA followed by SPSS.

Results: In our study Cells were treated with C.cassia bark extract 20 μg/ml for 24 h along with control group. These results revealed that C.cassia bark inhibited the proliferation of MCF-7 cells, changed the cytoplasmic morphology, promoted the apoptosis of MCF-7 cells, reduced the invasion and migration ability of MCF-7 cells, and exhibited anti-breast cancer effects. Therefore C.cassia bark primary constituents are linked to apoptosis, invasion, and metastatic targets in breast cancer cells. Overall the results of this study suggest that c.cassia extract could be employed as a possible anti- tumor agent.

Conclusion: As a result of the findings, Cinnamomum cassia was discovered to be cytotoxic to breast (MCF-7) cancer cell lines and also inhibits migration of breast cancer cells .Further invivo investigations and clinical trials may be conducted in order to develop c.cassia extract into a useful medication for treating breast cancer cells and make it commercially available.