Comparative Analysis of Time-Dependent Optical Density Based Microbial Load on Shrimp Samples Utilizing Colorimetry and UV-Vis Spectroscopy for Potential Food Pathogen Assessment

Abstract

Aim: To compare time dependent optical density based microbial growth on shrimp sample using Colorimetry and UV-Vis Spectroscopy for potential food pathogen analysis. Materials and Methods: In this study, Group 1 is OD from Colorimetry and Group 2 is OD from UV-Vis Spectroscopy. Sample size per group is 14 and the total sample size is 28. Shrimp samples were collected from local vendors and homogenized with saline. Spectroscopic wavelength was set to 600 nm and optical density readings were taken. Sample size was calculated for pre test power 80%. SPSS was used to determine the significance of this study using an independent sample T Test. Results: UV-Vis Spectroscopy obtained a higher mean OD of 0.39 whereas colorimetry obtained a lower mean OD of 0.28. Study shows a statistically  insignificant difference between the two groups with a  p value of 0.635 (p>0.05). Conclusion: UV-Vis Spectroscopy performed insignificantly better microbial load analysis on shrimp samples when  compared to Colorimetry.