Comparing the Deodorization Efficiency of Saccharomyces Cerevisiae and Lactobacillus Caseiin in Biodegradable Waste

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Nafeesa Kulsoom S, S Suji

Abstract

Aim: In this research, the potential application of Saccharomyces cerevisiae is compared with Lactobacillus casei for its novel deodorization capacity on 6 different types of waste samples. Materials and methods:Saccharomyces cerevisiae and Lactobacillus caseiwere used to treat waste samples. Nessler’s reagent was used to estimate the ammonium nitrogen present and UV-visible spectroscopy was used to calculate the percentage of ammonium nitrogen reduced after treatment in both the groups. Using clinicalc.com, sample size was determined as N=24 for each group utilizing the existing literature with an alpha error-threshold of 0.05 and 95% confidence interval, G power of 80%, and an enrolment ratio at 1. Results: The results show that S. cerevisiae reduced the amount of ammonium nitrogen by 22.5%, 9.7%, 7.7%, 12.9%, 5.2% and 7%, where asL. caseireduced the amount of ammonium nitrogen by 11.7%, 8%, 5%, 11.3%, 6.1% and 3.8% for cattle waste, poultry waste, fish waste, manure waste, vegetable waste and mixed waste respectively. ANOVA using SPSS version 28revealed that both the pairs are statistically significant with p value <0.001. Conclusion: By treating different types of waste samples with S. cerevisiae and L. casei, the reduction of ammonium nitrogen was successfully evaluated and S. cerevisiae was found to be more prominent, suggesting that it could be used as a possible deodorizer in odor management.

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