Comparing the effectiveness of Lactobacillus casei and Escherichia coli in deodorizing biodegradable waste

Abstract

Aim: The potential application of Escherichia coli against Lactobacillus casei for novel deodorization capacity on six distinct types of waste samples are investigated in this study. Materials and Methods: Escherichia coli and Lactobacillus casei were used to treat waste samples. Nessler’s reagent was used to estimate the ammonium nitrogen present and UV-visible spectroscopy was used to calculate the % of ammonium nitrogen reduced after treatment in both the groups. Using clinicalc.com, sample size was determined as N=24 for each group utilizing the existing literature with an alpha error-threshold of 0.05, a 95% confidence interval, G power of 80%, and an enrolment ratio at 1. Results: The results show that E.coli reduced the amount of ammonium nitrogen by 0.9%, 2.2%, 11%, 15.2%, 0.8% and 0.6%, where as L.casei reduced the amount of ammonium nitrogen by 11.7%, 8%, 5%, 11.3%, 6.1% and 3.8% for cattle waste, poultry waste, fish waste, manure waste, vegetable waste and mixed waste respectively. ANOVA using SPSS version 28revealed that both the pairs are statistically significant with p value <.001. Conclusion: The reduction of ammonium nitrogen in waste samples was effectively tested by treating them with E.coli and L.casei. L.casei was discovered to be more prevalent, indicating that it could be used as a possible deodorizer in odour management.