Molecular Comparative Study Of Janus Kinase2 Expression In Breast Cancer Of Human And Mice

Abstract

Breast cancer is a multifactorial disease with the highest frequency in females. Genetic and environmental factors can cause mutation in several genes like tyrosine kinase, Janus kinases are a family of non-receptor tyrosine kinases involved in malignancies  and other disease , In breast cancer We aimed to investigate its role at transcriptome level and its relationship with the clinical outcome of breast cancer in human and mice , This study enrolled a total of 25 samples for women with breast cancer , on the other hand we induce mammary gland carcinoma in mice by chemical carcinogenic (MNU) treated.

Twenty –five BC patients were included in this study , for these patients , 25 blood sample were taken for molecular analysis, sequenced using sanger sequencing for  analyzing exon 9 of Jak2 gene  ,  in mice tissue section of mammary gland were taken  to DNA extraction for detection of mutations in jak2 gene

molecular analysis for women patient DNA extraction from blood using sanger sequencing , showed absence of  SNP rs77375493 V617F , others SNPs in jak2 gene was demonstrated for the first time  such as   (Rs1168791735 ) G/A , (Rs1489598500)A/G and (Rs 755212421)C/A , in mice there was no detection of  mutation by  using sanger sequencing.