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Narmada river water is used for drinking, domestic and for irrigation purposes in Gujarat, due to its wide application its water quality is an important aspect. In the present study water sample of Narmada River from five regions has been assessed twice a month to evaluate its suitability for drinking, domestic and irrigation purposes. The important parameters selected for this study are Temperature, Turbidity, pH, Conductivity, T.D.S, Suspended Solid, Alkalinity, Acidity, Total hardness, Calcium hardness, Magnesium hardness, Chloride, Dissolved Oxygen, B.O.D, C.O.D and microbiological parameters were determined in the laboratory. The Physico- Chemical parameters were determined as per standard methods. Analysis results showed that the overall water quality is suitable and safe for domestic and irrigation purposes.