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Seabuckthorn has attracted worldwide engrossment because of its high medical and nutritional potential. Seabuckthorn berries are prosperous in vitamins, minerals, fatty acids, amino acids, carotenoids and antioxidants. Multifarious chemical substances are found in seabuckthorn which has a good effect on health. In this research, we used Thermo Scientific Multiskan Sky Cuvette Touch Drop Spectrophotometer to probe the presence of antioxidant assay in seabuckthorn fruit pulp using DPPH [α,α-diphenyl-β-picrylhydrazyl] reagent. The IC50 value for ascorbic acid and fruit pulp were calculated and observed that the IC50 values for ascorbic acid and fruit pulp sample were 19.07 µl and 12.82 µl, respectively. The pulp possessed remarkable antioxidant properties due to its ability to scavenge free radicals.