Molecular Detection Of Toxoplasmosis In The Intermediate Hosts By Using B1 Gene Characterization

Abstract

The goal of this research was to detection of Toxoplasma gondii by the molecular technique and comparison with the conventional methods such as rapid test and identification the group of woman that more susceptible to infection. The study included 55 blood samples from pregnant women and 30 samples from small ruminants (sheep and goats) suspected with the infection Toxoplasma gondii during the period from January, 2021 to May, 2021, which was confirmed by a rapid test examination. The results was revealed that 34 (40%) positive samples and 51 negative samples, which were considered a control group. On the other hand, the results of PCR technique for detection of B1 gene revealed that 35(63.6 %) of samples were found positive, the primers that amplify the outer and inner primers that had molecular weight 580bp and 531bp respectively.