Green Synthesis: In-Vitro Anticancer Activity Of Silver Nanoparticles Against Mkn45- Human Gastric Cancer Cell Lines

Abstract

Silver nanoparticles (Ag NPs) were synthesized by a green route using an aqueous Phaseolus vulgaris (green beans) extract. Water extracts of leguminous have the latent to be efficient anticancer ingredients, by means of copious mechanisms based on the raw material and the process. The characterization was conducted using a UV/Vis spectrophotometer (Jasco -model UV- 730), FT-IR, SEM (FEI Quanta FEG 200-High Resolution Scanning Electron Microscope), EDXA and TEM (JEOL 2100F FEGTEM). The cytotoxicity of the sample on AGS cells were determined by the method of Mosmann, (1983). The IC50 values were calculated and analysed based on the number of cells which exhibited suppression in cell growth on administration of Phaseolus vulgaris derived silver nanoparticles. Measurement of apoptotic induction using Acridine Orange/Ethidium Bromide (AO/EB) dual staining method is permeable and it stains all the Gastric Cancer (AGS) cells viable/nonviable cells. Using fluorescent rhodamine 123 (Rh 123) dye, mitochondrial membrane potential (MMP) was determined in this work. Our experimental results show that the Ag NPs can induce apoptosis and suppress the proliferation of AGS cells.