Isolation and Characterization of Probiotic Strains from Garcinia mangostana (Mangosteen) for Nutraceutical Applications

Abstract

Introduction: The present study concerns the isolation and characterization of Lactic acid bacillus sp. (LAB), isolated form fresh Magosteen fruit pulp. Lactic acid bacteria sp. (LAB) are an important source of bioactive metabolites and enzymes. This research aimed to isolate and identify potential probiotic strains from Mangosteen, scientifically known as Garcinia mangostana, is a tropical fruit native to Southeast Asia and comprehensively characterize their functional properties for potential nutraceutical applications.

Methodology: Gram staining and biochemical analyses were conducted to assess the microbial characteristics and identify the isolates. The strains were further evaluated for their probiotic potential, including resistance to gastric acidity, bile tolerance, and adhesion to intestinal epithelial cells. The isolated strain was evaluated for isolation and purification on MRS plates, Gram staining, Catalase test, and morphological examination under microscope, Biochemical test such as IMVIC, starch hydrolysis, carbohydrate fermentation. Antimicrobial activity of cell-free supernatants from presumptive LAB isolates was evaluated by centrifugation against Gram-positive, Gram-negative, LAB, mold, and yeast strains.

Results: Thirty-seven colonies isolated and characterized from the culture plate and all were gram positive, catalse negative, and nine isolates showed the most potent lactic acid bacillus strain characteristics. Overall, Lactic Acid Bacillus sp. isolated from fruit showed potential technological applications and should be further evaluated.