Determination Of The Fertility Rate Of Frozen Thawed Kundhi Buffalo Bull Semen With Skimmed Milk And Tris Based Semen Extenders

Abstract

The present study explored the fertility rate of frozen-thawed kundhi buffalo bull semen with skimmed milk and tris based extenders. Total four healthy khundi buffalo bulls of 3 to 4 years of age were selected. After collection the semen was examined for volume, colour, pH, wave motion, motility, Sperm concentration, morphology, viability and membrane integrity.After initial examination semen sample having motility, morphology and viability ≥ 75 % were diluted in Tris based extender consist of 6, 8, 10, 12, and 14gm concentration of skim milk. After dilatation Post chilled semen characteristics result showed Motility was (58.55±5.47), morphology (66.75±3.30), membrane integrity (66.75±1.25) and viability (58.25±6.4). Frozen thawed result showed motility (54.75±4.03), morphology (64.75±2.50), membrane integrity (62.25±3.86) and viability (64.00±4.47). Skim milk treatment group conception rate is significantly higher 40% than tris base extender 20%.Overall, skim milk added groups showed improved frozen thawed quality of semen than control. However highest values were obtained in 10gm concentration of skim milk added in