Clinical application Protease from Acinetobacteria Species.

Salunke.S M; Dr. Jadhav B. N; Dr. Satpute. R. A.

doi:10.53555/sfs.v10i3.3238

Authors

Salunke.S M
Dr. Jadhav B. N
Dr. Satpute. R. A.

DOI:

https://doi.org/10.53555/sfs.v10i3.3238

Keywords:

Protease, Acinetobacteria species and anti-inflammatory and anti- cancerous activity.

Abstract

Extracellular protease from Acinetobacteria species isolated from fish gut. The protease was produced and purified by ammonium persulphate, dialysis, Sephadex G column chromatography and Ion Exchange Chromatography and HPLC. Characterization was done by using BAPNA substrate and MALDI-TOF. Purified protease was studied for anti- inflammatory and anti-cancerous activity. The 50 percent inhibitory concentration was as followed for IL-6 0.348 mg/ml and LNCAP cell line 30.11µg/ml.

Author Biographies

Salunke.S M

Department of Biotechnology, Vinayakrao Patil Mahavidyalaya, Art’s Commerce and Science College Vaijapur. Chhatrapati Sambhajinagar-423701, Maharashtra, India.
Dr. Jadhav B. N

Department of Zoology, Vinayakrao Patil Mahavidyalaya, Art’s Commerce and Science College Vaijapur. Chhatrapati Sambhajinagar-423701, Maharashtra, India.
Dr. Satpute. R. A.

Government Institute of Science, Department of Botany Chhatrapati Sambhajinagar-423701, Maharashtra, India.