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Using tornado plasma gliding arc discharge system of non-thermal atmospheric pressure that ran frequency 9.1(kHz), AC power supply high voltage 8 kV, argon gas flow rate of two liters per minute and use magnetic coil contains 500 turns and two electrodes. The current study aimed to detect the effect of tornado plasma gliding arc discharge on the bacteria. Use three types of bacteria is Staphylococcus aurous (Gram-positive), While salmonella and Escherichia coli (Gram-negative). Each type of bacteria is subdivided into six groups one control and five treated with tornado plasma gliding arc discharge for times (10,15,20,25, and 30 min). The results of the study revealed a large rise (P<0.05) in the rate of killing of bacteria treated with tornado plasma compared with control group. It can be noted that increasing the exposure time leads to a decrease in the number of bacteria colonies.