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From (55) soils samples were collected, including soils from different regions of Nineveh and Duhok governorates, of which (29) isolates were isolated, with a rate of (52.7%) belonging to the genus Bacillus. Eleven (11) bacteriocin-producing isolates were selected, which were diagnosed as Bacillus clausii-AE, Bacillus subtilis-AE, Bacillus cereus-AE, Bacillus thuringiensis -AE, Bacillus sp. -AE depending 16S rRNA gene sequences. The Bacillus species clustered in 5 clusters: A: include 5 strains B. subtilis-AE at 100%, B: include 2 strains
B.cereus-AE at 99.8%, C: include 2 strains B. clausii-AE at 99.6%, D: include 1 strains B. thuringiensis-AE at 99.2%, E. include 1 strain Bacillus sp.-AE at 94.4%. Inphylogenetic tree using Unweighted Pair Group Method with Arithmetic mean (UPGMA), using (Mega 7) program. The Bacillus sp.-AE has the highest effect on the bacterial type Acinetobacter junii -20AE, Acinetobacter baumannii- 19AE, Acinetobacter baumannii -22AE. The bacteriocin extracted from Bacillus sp.-AE was identified as a Mersacidin type Lantibiotics, which is a protein complex consisting of a peptide, the Rf value is 0.70 by TLC method, and the molecular weight is less than 5 kDa by SDS method. -PAGE GEL.