Evaluation of Hepatoprotective and Polyherbal Formulation for Methotrexate Induced Toxicity Inalbino Wistar Rats

Abstract

Herbal formulations means dosage form consisting of one or more herbs or processed herbs in specified quantities to provide specific nutritional value, treatment and mitigate diseases. Vitis viniferathe fruit is used as food supplement and the seeds and leaves are employed in herbal therapy. The present study was carried out to evaluate the hepatoprotective activity role of seed extracts of Petridafoetida and Vetisvenifera. Hepatoprotective activities of ethanolic and aqueous extracts of Vitisvinifera were examined against methotrexate induced liver damage in waster rats using silymarin as control. Enzyme activities of Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT) and Alkaline Phosphatase (ALP) were analyzed. Petridafoetida and Vitis venifera seeds extract of exhibited significant (P<0.05)hepatoprotective activity. Ethanolic seeds extract ofVitis vinifera and Petridafoetida exhibited moderate activity over methotrexate treated animals. Results confirm the traditional - ethno medicinal use of methotrexate as a potential source of hepatoprotective activity.