Hptlc Finger Print Profile Of Steroids From Hydroalcohol Extract Of Leaves of Holoptelea Integrifolia (Roxb.)

Abstract

Holoptelea integrifolia belongs to the family ulmaceae commonly called as Indian Elm and commonly used in India by the tribal people for it’s medicinal properties. Plants and plant-based products are the basis of many of the modern pharmaceuticals we use today for various ailments. High performance thin layer chromatography (HPTLC) is a valuable tool for the investigation of herbal products with respect to different aspects of their quality.

 High Performance Thin Layer Chromatography (HPTLC) technique was utilized to find steroids from hydroalcohol exrtact of therapeutically and commercially beneficial leaves of Holoptelea integrifolia Phytochemical screening was performed as per standard procedure followed by HPTLC analysis. HPTLC instrumentation of CAMAG was having Linomat V applicator (Switzerland). CAMAG Thin Layer Chromatography scanner IV with reflectance absorbance mode at 208 nm , Win CATS software (1.4.6 Camag) and tungstant lamp was utilized to perform densitometric scanning.

Hydroalcohol extract of Holoptelea integrifolia appeared to give the presence of steroids, tannins, alkaloids, glycosides, flavonoids, phytosterols from preliminary phytochemical analysis. HPTLC studies from hydroalcohol extract of leaves revealed nine multivalent phytochemicals (9 peaks) having Rf values in the increasing range of 0.09 to 0.50 Rf 0.44 has 48.97% concentration.

Preliminary phytochemical analysis and Rf Values authenticated existence of steroids in hydroalcohol extract