Insecticide With Active Methomyl Ingredients Against Hematological Parameters As Information on Stress Conditions of Tilapia Strain Jatimbulan (Oreochromis niloticus)

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Nurhalisa, Diana Arfiati, Sri Andayani, La Ode Baytul Abidin, Vina Nur Nadiro

Abstract

Methomyl is used to control and eliminate pests (insects) in agriculture. Methomyl residues have been detected in agricultural products and natural waters. The purpose of this study was to analyze the effect of exposure to insecticides with the active ingredient methomyl with different concentrations on hematology (erythrocytes, hemoglobin, hematocrit, leukocytes) as information on stress conditions for Tilapia Jatimbulan which were observed on the 4th day after the first exposure and the 4th day after the exposure 7th (end of study 28 days). The study used a completely randomized design with 1 control without exposure and 3 exposure treatments namely concentrations of 1.8 mg/L (Treatment A), 4.015 mg/L (Treatment B), 6.2 mg/L (Treatment C), with each 3 repetitions. Six tilapia fish measuring 9-12 cm and weighing an average of 30.54 ± 1.17 g were put into an aquarium (30 L) containing fresh water and insecticide with the appropriate concentration of treatment. Four days after the first exposure, one fish was taken from each aquarium to be analyzed according to the observed parameters. Then the fish were temporarily moved (± 30 minutes) to another aquarium, the treatment aquarium was cleaned and filled with insecticide again with the concentration according to the treatment then the fish were put back in for (second) exposure for 4 days. The treatment was repeated every 4 days until the 7th exposure (28th day). Insecticide Lannate® 25 WP which was used for detection by FTIR and GCMS, is known to contain 32.55% methomyl content. Tilapia experienced stress due to hypoxia, after the first exposure (day 4), marked by increased hematology according to the increase in insecticide concentration. After 7 exposures, hypoxic stress still occurred in treatment C, marked by an increase in hematology. It is know that the concentration of insecticide exposure of 6.2 mg/L resulted in fish experiencing severe stress up to 7 times the exposure (28 days at the end of the study).

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