Therapeutic effects of Mangifera indica extract against Proteus mirabillis isolated from dogs with UTI, Invivo and invitro study

Abstract

The aim of this study was for detection the therapeutic effects of Mangifera indica extract against Proteus mirabillis isolated from dogs with UTI, Invivo and invitro study. Fresh Mangifera indica were purchased from local market in Fallujah city. Plant shell were washed under tap water, and then dried at room temperature at shade. The dried shell was crushed by a laboratory blender. Organic solvents were used for the  extraction of Mangifera indica shells by using absolute ethanol (Ethyl alcohol) which was considered as very effective in extracting the active ingredients of the plant according to the method described by many authors previously. Analysis of Mangifera indica by HPLC for detection of active ingredients. Determination the activity of Mangifera indica in vitro was done by using Agar well diffusion method was used to check the activity of plant extract in vitro for 3 concentration (100,150,200). Induction of infection in mice and Invivo study done on sixty (60) Mice were selected for infection which divided into 3 groups (each group contain 20 animals),Infected group (control positive) with dose 2×108 CFU/ml, Infected group with dose 2×108 CFU/ml then treatment with Mangifera indica extract at dose of 200mg/ml, control negative group which given only normal saline. Three concentrations of extracts were used for determination activity of M. indica, 100,150,200 mg/ml were used. The results of HPLC showed that analysis of extracts contains Qurcetine , Gallic acid, Rutin, Kaempferol, Apigenin,Catechin. The main component were Gallic acid as well as Qurcetine. A concentration of 200 mg/ml were showed to be more active against bacteria. The present results of invivo study revealed that mice in G1 exhibited signs of UTI which diagnosed by isolation of bacteria during all the period of experiment, while mice in G2 showed that decrease of bacterial count till its subsids after 2 weeks of treatment by Mangifera indica.

In conclusion, Mangifera indica have antibacterial effects against virulent Proteus mirabillis isolated from dogs with UTI.