Detection hla and spi gene of Staphylococcus aureus and it is relation with agr system presence

Abstract

Background: Staphylococcus aureus genomes size are almost 2.8 Mbp , these genomes are having a very similar architecture and differ in their function like accessory genome that encoded to virulence factors of Staphylococcus aureus pathogen’s caused broad range of diseases .

Methods:  Identification of Staphylococcus aureus bacteria that isolated from urine specimens have been done according to the cultured on the mannitol salt agar and biochemical tests, then confirm the identification by amplification 16srRNA gene, virulence factor gene hla and sbi genes amplification by conventional PCR and agr gene amplification by multiplex PCR

Result: Thirty isolates of Staphylococcus aureus diagnosed and found all this isolates have agrI,II,III,IV  that related with hla gene and sbi gene

Conclusion: Urinary tract system was more infected with S. aureus bacteria and agr system was regulated virulence gene ( hla gene and sbi gene).