Isolation and identification of Multidrug-Resistant (MDR) bacteria from ophthalmic patients in some hospitals in Basrah

Abstract

104 samples of patients with eyes inflammation were collected from some hospitals and outpatient clinics in Basrah Province, 44 samples of males, at a percentage of 42%, and 60 samples of females, at a percentage of 58%, and they were of different ages. We found 74 developing bacteria samples (positive growth) at a percentage of 71%, and 30 samples with no bacterial growth at a percentage of 29%. 12 multi-drug resistant (MDR) bacterial samples were diagnosed, at a percentage of 16% of the total positive growth bacterial samples, represented by Staphylococcus aureus (50%), Escherichia coli (25%), Acinetobacter sp. (16.6%), and Proteus mirabilis (8.3%). The study revealed the resistance of all isolated samples of S. aureus bacteria to multiple antibiotics such as Trimethoprim, Methicillin, Cefoxitin, and Cefixime. Gram-negative bacterial isolated samples showed resistance to Cefixime, Trimethoprim, Aztreonam, Amoxicillin clavulanic acid, Gentamicin, and Tetracycline in varying proportions. The study also revealed the sensitivity of all Gram-positive bacterial samples to Ciprofloxacin, Chloramphenicol, Gentamicin, Vancomycin and Azithromycin antibiotics. It further indicated that some Gram-negative bacterial samples are sensitive to Piperacillin, Meropenem, Ciprofloxacin, and Chloramphenico.