Effect of Testicular injection of Autologous Platelet Rich Plasma (PRP) on Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone hormone in goat

Abstract

The present study has been designed to investigate wheather PRP effect on semen of goats. The study has been conducted on adult male goat at the, Al-Qadisiya Gouvernante Al Muhanawiah locality  during the period extended from March 3 2022, to June 2 2022. Male goats were assigned to 5 equal groups and treated as follow:  injected with PRP (0.25 ml), T2: injected with PRP (0.50 ml), T3: injected with PRP (0.75 ml), T5: just injected without any things. The blood samples were obtained from jugular vein in non-heparinized tubes and PRP tubes. Blood serum samples were separated (by centrifugation at 3000 rpm for 5 minutes) and kept at –20 ºC until assessment of LH, FSH and Testosterone concentrations. The results of this study demonstrated there was a significant increase (p<0.05) in LH, FSH and Testosterone concentration after treated with PRP in T1, T2 and T3 groups as compared with T4 and control groups) in 10th week, while there was a best result and a significant increase in LH concentration in T2 group as compared with T1, T3, T4 and control group in 10th week.  As well as the results showed a significant increase (p<0.05) in LH concentration after treated with PRP in T1, T2 and T3 groups as compared with T4 and control groups in 12th week, while there was a best result and a significant increase in LH concentration in T2 group as compared with T1, T3, T4 and control group in 12th week. Whereas the results showed a significant increase (p<0.05) in LH concentration after treated with PRP in T1, T2 and T3 groups as compared with T4 and control groups in 14th week, while there was a best result and a significant increase in LH concentration in T2 group as compared with T1, T3, T4 and control group in 14th week. And there was non-significant difference (p > 0.05) between Control and T4 groups. As for the time periods from the first week to the third week, they were without injections of anything, and there were no significant differences between the values for all groups. As for the 10th week, the 12th week, and the 14th week, there were significant differences between them in terms of values, especially in the T1, T2 and T3 groups, compared with the control group and the T4 group.