Utilization of Some Agro Wests for the Production of Acid Protease by Aspergillus niger

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Belan Othman, Nawal H. Sebo

Abstract

Acid protease production under solid-state fermentation was investigated using isolated Aspergillus niger, which produced a higher amount of extracellular acid protease (130 U/ml) than other locally isolated fungal specie under submerged liquid fermentation. Among all agro-industrial waste materials evaluated, dried melon peel supported maximum protease production. The addition of carbon and nitrogen sources to the solid medium increased the yield and synthesis of enzymes. Using casein as a nitrogen source and fructose as a carbon source, maximum enzyme synthesis was accomplished, increasing enzyme activity to (750U/gm). The synthesis of acid protease was greatly influenced by physiological fermentation variables, with 105 (spore/ml) being the ideal inoculum size. These factors included the pH of the medium (pH 6.0), moisture ratio (1:0.75), incubation duration (120 h), temperature (35 °C), and inoculum level.


 

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Belan Othman, Nawal H. Sebo