Synthesis of novel 2-chloro-6-{[(E)-{2-hydroxy-3-methoxy-5-[(E)-1,3-thiazol-2-yldiazenyl]phenyl}methylidene]amino}-4-nitrophenol and its Co(II) Cu(II) complexes : biological studies and Detection of Hg2+ by [Co(CEN)2]/GCE

Abstract

In this study, the novel azo-dye 2-chloro-6-{[(E)-{2-hydroxy-3-methoxy-5-[(E)-1,3- thiazol-2-yldiazenyl]phenyl}methylidene]amino}-4-nitrophenol (CEN/C₁₇H₁₂ClN₁₀O₁₀S) and Co (II), Cu (II) complexes were synthesized. The structure of synthesized metal complexes and CEN     were characterized by physico-chemical technique by FT-IR, elemental analysis, UV-Vis, mass spectra, ¹HNMR spectra and thermogravimetric analysis; all spectral data suggests trident ligand and prepared complexes shows octahedral geometry with PXRD exhibit crystanality nature of complexes. Ligand and its [Co(CEN)2], [Cu(CEN)2] were tested for their in vitro antimicrobial and antioxidant properties. The [Co(CEN)2] act as a good antioxidant agent. In this connection, we designed a new sensor for electrochemical determination of Hg²⁺ by CV. The GCE was modified with [Co(CEN)2] to enhanced, because the electron transfer behavior of the electrode. The modified [Co(CEN)2]/GCE has long linear range of 10-70 µM/L,  sensitivity of 4.059 μAμM⁻¹cm^-2 and LOD (limit of detection) is 3.333 µM/L respectively in PBS  pH 7.0. The modified biosensors is monitoring the mercury in aqueous water and recovery result obtained. Prepared sensor [Co(CEN)₂]/GCE has more stability and showed less leaching property.