The Association of single nucleotide polymorphism of IL4 with serum level of anti-Hepatitis B Antibodies

Abstract

Hepatitis is a liver infection and inflammation that can be acute or chronic with a diverse etiology, including viruses, bacteria, medicines, and hazardous chemicals.Hepatitis B  is the most common causes of chronic and acute  hepatitis disorders worldwide. A cross-sectional study was carried out in Salahaldeen governorate from 15th of December 2021 to 25th of Augest2022.The study included 90 hepatitis patients with hepatitis B ranging in age from18 to 61 years.These individuals were hospitalized to general  Sammarra hospital. The control group, which was matched to the patients analyzed, consisted of 30 people who appeared to be healthy.5ml of blood was taken from each patient in this study for the purpose of ELISA testing to check HBs Ag,antibodies to HBs and molecular test to IL4 by pcr.                                                                 

The result showed that all patient with hebatitis B had HBsAg and the number of people with HBV in males more than female the frequency distribution of HBsAg did not differ significantly between males (60 %) and females (40 %) .The study included investigation the polymorphism of IL4 genes for patients which infected with HBV and healthy control,and it included (C-589T (rs2243250) , T + 2979G (rs2227284). For T + 2979G (rs2227284) it was found that there was a significant increase in p-value (p 0.05 ) for the genotype(GG,GT,TT) when comparing patients with control.This indicates that the genotype GG causes the risk of developing hepatitis B and the frequency of the T allele for healthy is the highest compared to patients .For C-589T (rs2243250) it was found that there was homzygous mutant genotype (TT) was more frequent in HBVpatients than controls (20% vs. 16.67%), the difference was not signifcant.  Likewise, there was no signifcant difference between patients and controls in allele frequency. Furthermore, neith er dominant nor recessive inheretance model had a signifcant difference between the two group. On the other hand, the homozygous genotype (CC) was more common among patients  than control (43.33% vs 40 %) with no significant difference. The mean serum level of IL-4 in patients carrying TT genotype of rs2227284 was 92.24±71.25 pg/ml which significantly higher than those carrying GG genotype (50.96±13.6 pg/ml) or those carrying GT genotype (47.74±9.6 pg/ml). Although, patients carrying the TT genotype also had higher level of anti- HBs IgG level than either those carrying GT or GG genotypes, the differences were not significant.For the rs2243250, the different genotypes associated with comparable levels of IL-4 and anti-HBs IgG with no significant differences.Incontrol group Persons carrying the TT genotype had also higher level of IL-4 and anti-HBs IgG (92.15±18.69 pg/ml and 16.21±5.72 U/L, respectively) than those carrying GG genotype (81.55±16.61 pg/ml and 12.48±4.54 pg/ml, respectively) or those carrying GT genotype (70.05±7.4 pg/ml and14.26±6.12 U/L, respectively); however, the differences were not significant.