Effect of Nano-extract of Silybum marianum seeds on liver enzymes and blood proteins in male albino rat induced liver cancer

Abstract

The current study aimed to find out the protective role of ZnO nanoparticles using Silybum marianum seeds on rats induced liver cancer by measuring some liver enzymes and blood proteins. In this study, Twenty five male white rats were used and divided into five groups. The first group, G1, was considered a negative control group, and the second group, G2, was considered a positive control group they were injected subperitoneal with thioacetamide (200mg/kg) for 14 days, then they were injected with CCl4 (3mg/kg). Once every week till the end of the experiment, while the remaining three groups were dosed with nano-extract at concentrations (15.8.5 mg/0.250kg) for 14 days, then injected subperitoneal with thioacetamide for 14 days, then injected with CCl4 once every week till end of the experiment. The study lasted for14 weeks. Blood samples and liver tissues were collected and the liver enzymes AST, ALT, ALP, albumin, globulin, and total protein were measured, the following results were discovered: A significant increase (P<0.05) in the level of the three liver enzymes (AST, ALT, ALP) for the positive control group compared to the negative control group. Also, the study showed that there were no significant differences (P>0.05) between the third group and the positive control group, but there was a decrease in significant level of liver enzymes in fourth and fifth groups comparing to the positive control group, and a significant decrease in the level of albumin, globulin and total protein of the positive control group comparing to the negative control group, but a significant increase occurred in groups G3, G4 and G5. Also the study showed that there were histological damage in G2 compared to the control group, but the damage was less in the other three group (G3,G4,G5) comparing to the positive control group .