Isolation, Identification, and Bioactivity Test of Secondary Metabolite Compounds from Methanol Extract from Java Bark

Abstract

This study aims to isolate, identify, and test the bioactivity of secondary metabolites of lannea coromandelica stem bark methanol extract. This type of exploratory research included the stages of fractionation, purification, identification, and bioactivity testing of the methanol extract of lannea coromandelica stem bark. The sample used came from Tondong Bua Village, Bone District, Indonesia. The pure isolate obtained was in the form of a white powder. Identification results with TLC Three Eluent System obtained Rf 0.2, 0.53, and 0.85, respectively. FTIR spectrum data shows the presence of secondary N-H groups at wave numbers 3446.79 cm-1, C-N at wave numbers 1205.51 cm-1 to 1302.74 cm-1, C=C Aromatic at wave numbers 1577.77 cm-1 to 1643.36 cm-1 and C-H Aromatic at wave number 690.52 cm-1, melting point 156-158°C. Based on the group test results, FTIR spectral data, and GC-MS, it was concluded that the compound obtained was an alkaloid compound, namely 2,5-dimethyl pyrazine. The results of the bioactivity test on Artemia salina Leach larvae for the crude extract of the GF faction and the pure isolate showed LC50 values of 13, 15, and 36.41 ppm, respectively, indicating that the crude methanol extract obtained had higher bioactivity compared to the pure isolate. But both have high potential as anti-cancer and anti-microbial.