Phylogenetic analysis and comparative investigation in Al-Diwaniyah City and worldwide isolates from genebank for Shigella species

Abstract

Between November 2020 and May 2021, 30 samples from diverse patients admitted to the general hospital, the teaching hospital for women and children, and the Al-Diwaniyah teaching hospital yielded a total of 20 Shigella species isolates. The isolates, numbered No.1 to No.8, were identified using standard methods and the polymerase chain reaction methodology. The entire genomic DNA of twenty isolates, numbered 1 to 8, isolated from various geographic locations of Iraq, was retrieved. Pathogenic Shigella spp. 16S ribosomal RNA gene sequencing was done, and the locations of these genes on the isolates' genomes were determined.

Sequencing of the 16S ribosomal RNA gene from pathogenic Shigella spp. was done, and the locations of these genes on the isolates' genomes were identified. Using the MEGA 6 software, a phylogenetic tree analysis was completed, and the local Shigella spp. was revealed by the comparison of the current study isolates with the worldwide Shigella strains in the NCBI-Gen bank.