Antibacterial activity of protein of marine fish Sphyraena putnamae (Jordan and Seale, 1905) of Gulf of Mannar, south eastern India

Abstract

Fish is an important and cheap source of essential nutrients. Protein of Sphyraena putnamae was extracted and the crude fish protein was partially purified by dialysis and quantified by Bradford analysis against the BSA protein standard. The concentration of the partially purified protein of S. putnamae was estimated to be 2.00±0.75 mg/g. The antimicrobial activity of the fish protein was studied by agar well diffusion method. The protein sample at different concentrations inhibits bacterial growth and the study recorded the zone of inhibition against Streptococcus oralis and Streptococcus pyogenes.  According to the results, the protein sample exhibits concentration-dependent inhibition against bacterial species. The maximum inhibition was observed at 500µg/ml and the zone of inhibition was 15.00 ± 2.00 mm against Streptococcus pyogenes and 11.25 ± 0.35 mm against Streptococcus oralis. This study demonstrates that the protein of Sphyraena putnamae possessing antibacterial activity can be extracted, identified and further developed into new pharmacological products.