Test of Zantac Mutagenicity on E. coli strain

Abstract

Results of this study were summarized by finding Minimum inhibitory concentration (MIC) of 8-azaguanine and zantac on the locally isolated normal flora strain E. coli that was identified by vitek-2 and 16s RNA .  MIC of 8-azaguanine was reached 500 µg / ml comparison with MIC of zantac which was 2250 µg / ml. The ratio of resistance to 8-azaguanine was increasing in a direct proportion to the increasing concentration of zantac that was added to media. The resistance of bacteria reached 82% at the concentration of 1500 µg / ml of zantac. Furthermore testing the mutagenicity of zantac by antibiotics was showed that zantac resistance wasn't affected by trimethoprim-sulfamethoxazole (SXT) and azithromycin. Whereas sensitivity of E.coli was decrease towards amikacin and gentamicin, (aminoglycosides antibiotics) was decreasing during the increase of zantac concentration this gives evidences the zantac mutagenicity on DNA or RNA molecule by increasing its concentration on E. coli in the current study.