Assessment of Acute Toxicity, Median Lethal and Safe Dose of Hexaconazole to an Indian Air-breathing Murrel, Channa punctatus (Bloch, 1793)

Abstract

The ecotoxicity of Hexaconazole for Channa punctatus (Bloch) is reported in this paper. During 2023, the work was carried out using the static renewal method. The 96hr-LC50 value from Lorke and Enegide et al, method was estimated to be 58.09 and 60.0 mg/L in this experiment. As a rough estimate, the Up-and-Down method yielded a 96hr-LC50 value range of 47.5 to 57.5mg/L. Furthermore, the 96hr-LC50 values for Behren-Karber, Miller and Tainter and Finney methods were 76.5, 76.203, and 85.11mg/L, respectively. Finally, the Reed-Muench method was used to confirm the 96hr-LC50 dose and a median ideal dose of 68.61mg/L was discovered. In Channa punctatus, the cumulative median lethal average of 96hr-LC50 dose was 76.500+76.203+85.110+68.609 = 76.6055 mg/L of Hexaconazole. The dose of Hexaconazole and the mortality offish showed a significant relationship.  The findings indicate that Hexaconazole accumulates in aquatic bodies, disrupting the physiological activities of this fish.

It can be inferred that this fish has a greater ability to resist Hexaconazole toxicity than other fishes. The value of the calculated safety level indicated that it is feasible to determine an acceptable dose of Hexaconazole for this fish. Based on traditional toxicity, Hexaconazole may even be classified as a moderately/highly cytotoxic substance to Channa punctatus.