Application of INCOMYCIN Against Induced Skin Infection in Mice by METHICILLIN RESISTANT STAPHYLOCOCCUS (MRSA)

Abstract

Skin and soft tissue infections are the most prevalent and frequent form of Methicillin-resistant Staphylococcus aureus (MRSA) infections. MRSA is characterized by resistance to most ß-lactam antibiotics. It can cause various infections including endocarditis, supportive pneumonia, and osteomyelitis. This study evaluates lincomycin activity against infection models induced by MERSA in induced neutropenic mice. The bacterium was isolated from one hundred and fifty samples of infected dog ears' exudate discharge from different locations in Baghdad governorate, Iraq during the period from October 2021 to march 2022, isolate achieved a probability of 99% belonging to Staphylococcus aureus by VITEK® 2 system identification The isolates showed high rate  (75%) sensitive to Lincomycin, Mice rendered neutropenic by a total dose of cyclophosphamide 250 mg/kg intraperitoneal injections , Induction of Skin infection model by utilizing of a certified S. aureus isolate and assessing the incidence of infection through the development of clinical signs, inflammatory cell counting and counting of the recovered bacterial colonies. The treatment with Lincomycin (22mg/kg q 12 hrs.) orally was started immediately after diagnosis of the infection, and the bacteriological cure was assessed every 48 hrs. Lincomycin showed effective and satisfactory results within one week of treatment.