Enterobacter cloacae identification by 16S rRNA and rpo B gene sequencing of the Cichlidae Tilapia fish pathogen in Babel local market

Abstract

        This study was carried to investigate the presence of Enterobacter cloacae pathogen in fish Tilapia (n = 21), Liza abu (n = 9). A total of 30 samples from different region of fish, routine bacteriological examination were done and evaluation of isolates by PCR assay using of 16S rRNA and rpo B genes, The results was showed that 11 Enterobacter cloacae  isolates tested and produced bands with the same intensity as the standard PCR performed using pure DNA. The genetic methods allowed detecting the virulence factors of bacteria. According to 16S rRNA sequence comparisons, the type strain of E. cloacae showed closely related to Enterobacter species similarity levels (97.8%) when compared with references strains in Genbank. Phylogenetic tree was distributed by neighbor- goining method appeared closely related to E. cloacae.  The results of the present study constitute an advance in the available diagnostic methods for bacterial pathogens in fish markets.