Anticancer And Antitubercular Activities of Hexane Compounds from Toona Ciliata Leaves

Abstract

The emergence of resistance had made the tuberculosis treatment difficult and many lives are lost due to this. Severe toxicities are reported with the use of the anti cancer drugs. All these problems have lead to the search for the new lead molecules that can be safely used in the treatment of infections and certain diseases. Many biological activities have been reported with crude extracts of Toona ciliata. Leaves of Toona ciliata had been selected, since it is renewable source. We were able to isolate four compounds from the hexane extract of the plant. All the four compounds were testes for anti-tubercular activity by luciferase reporter phage assay.  Among the four, compound H4 was active against all the senisitive, resistant and standard strains of Mycobacterium tuberculosis. Compound H3 was active against resistant and standard strains of Mycobacterium tuberculosis. The anticancer activity was tested using MTT (3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and Annexin V/ Propidium Iodide staining using Fluorescence-activated single cell sorting (FACS) analysis. All compounds have shown good activity in the MTT assay and compounds H4 and H3 have shown more apoptotic activity