Study of Genetic Polymorphism Using ISSR Markers in the Lentil Collection

Abstract

Genetic diversity of the 46 introduced lentil genotypes was studied using ISSR markers. As a result, in the lentil collection was recorded high polymorphism (76%). The highest polymorphism was recorded in UBC 810 and UBC 809 (> 85.7%) primers. A total of 69 fragments were obtained using 9 ISSR marker and 75,3% of them were polymorphic. The average genetic diversity index  ranged between 0.54 to 0.81. Cluster analysis accessions were grouped into 6 clusters, Flip 2010-96 and Flip 2011-41, Flip 2011-32 and Flip 2011-97, ILL 10932 and Flip 2011-20, Flip 2010-81 and Flip 2011-19 were evaluated as the most distant genotypes.  The results can be recommended  for the breedig, cultivation and protection of lentil crop  in the future.