Molecular and phylogenetic analysis of Staphylococcus aureus isolated from feline otitis externa

Abstract

The study was conducted for the estimation of feline otitis externa caused by Staphylococcus aureus in Baghdad, a province of Iraq. One hundred and twenty ear swabs from cats with otitis externa were used for isolation of Staphylococcus aureus, after culturing on selective media Blood agar, Mannitol salt agar and Hi chrom agar, Gram staining and biochemical tests. The (n = 7) Staphylococcus aureus isolates were obtained from ear swabs of cats and confirmed by using polymerase reaction chain (PCR) for 23S rRNA and sequencing of this gene. Sequencing has been done in Korea by Macrogen and the evolutionary tree was drawn by using Molecular Evolutionary Genetic Analysis (MEGA). The results showed that the (7) isolates possessed the 23S rRNA gene (100%) by using specific primers at 350bp. The results of the phylogenetic tree revealed that the sequence of the (7) isolates revealed 99% compatibility with reference isolates at Gene Bank. It had been deposited under accession numbers (ID: ON908999.1, ID: ON909000.1, ID: ON909001.1, ID: ON909002.1, ID: ON909003.1, ID: ON909004.1, ID: ON909005.1)