Bacterial and Molecular detection of Pseudomonas aeruginosa in feline otitis externa in Baghdad city

Abstract

The aim of this study was toward isolation and identification of Pseudomonas aeruginosa from cats with otitis externa in Baghdad city Using culture, VITEK 2 SYSTEM and Polymerase chain reaction. Pseudomonas aeruginosa was isolated from eight cases of feline otitis externa (8%) from a total number of 100 cats, the clinical signs of otitis externa were Head shaking, scratching, excessive ear wax, malodor, Pain during palpation, Alopecia and Pus and/or blood. Culture was done on pseudomonas agar with cetrimid and it revealed eight positive samples with production of greenish-blue pigmentation of pyocyanin produced by the bacteria some isolates showed fluorescence (greenish-yellow) pigmentation. VITEK 2 SYSTEM identified six isolate as Pseudomonas aeruginosa,, on contrary, polymerase chain reaction revealed that the eight isolates were Pseudomonas aeruginosa using specific primers for pseudomonas aeruginosa identification like: gyr b190 and gyr b222 and the result was positive for the eight isolates. We discovered by using VITEK 2 SYSTEM for antibiotic susceptibility test that the isolates were 100% resistant to cefazolin and Tigecycline. This study demonstrated the percentage of otitis externa in cats, according to gender females were significantly higher than males. According to breed, Himalaya and Persian breeds had the highest rate of infection with otitis externa, according to month December and January(cold months) had higher rate of infection.